Brain angiotensin converting enzymes: Evaluation using mass spectrometry and Western blot.

A rapid assay for angiotensin-converting enzyme activity using ultra-performance liquid chromatography-mass spectrometry.

Angiotensin-converting enzyme (ACE) plays an important role in the renin-angiotensin system and ACE activity is usually assayed in vitro by monitoring the transformation from a substrate to the product catalyzed by ACE. A rapid and sensitive analysis method or ACE activity by quantifying simultaneously the substrate hippuryl-histidyl-leucine and its product hippuric acid using an ultra-performa...

A Comparative Study of qPCR, Western Blot and Mass Spectrometry for the Estimation of Protein Concentrations

It is of great biological and clinical value within proteomics to be able to accurately quantify proteins in a complex sample. However, this is a challenge due to the large dynamic range of protein concentrations, demonstrating the need for advance quantification techniques. The mass spectrometryand affinity-based techniques with their respective reagents need to be both sensitive and specific,...

Angiotensin-converting enzymes modulate aphid–plant interactions

Angiotensin-converting enzymes (ACEs) are key components of the renin-angiotensin system in mammals. However, the function of ACE homologs in insect saliva is unclear. Aphids presumably deliver effector proteins via saliva into plant cells to maintain a compatible insect-plant interaction. In this study, we showed that ACE modulates aphid-plant interactions by affecting feeding behavior and sur...

Angiotensin Converting Enzyme in Sarcoidosis

Application of liquid chromatography-electrospray ionization mass spectrometry for study of steroid-converting enzymes.

A high-performance liquid chromatography-atmospheric pressure ionization-electrospray ionization mass spectrometry (HPLC-API-ESI-MS) method was developed for the analysis of steroids in a study of steroid-converting enzymes. Separations ware done on a Zorbax Eclipse XDB-C18 column (eluted with a linear methanol-water-acetic acid gradient) and identification of the steroids involved was done by ...